Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid
نویسندگان
چکیده
منابع مشابه
Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid.
Gonadotrophin surge attenuating factor (GnSAF) is a new non-steroidal ovarian substance, different from inhibin, which attenuates the pre-ovulatory luteinizing hormone (LH) surge in superovulated women. Human follicular fluid (FF) was used as a source for the isolation of GnSAF, the activity of which was monitored in an in-vitro pituitary bioassay. Primary rat pituitary cells were incubated wit...
متن کاملSelection of gonadotrophin surge attenuating factor phage antibodies by bioassay
BACKGROUND We aimed to combine the generation of "artificial" antibodies with a rat pituitary bioassay as a new strategy to overcome 20 years of difficulties in the purification of gonadotrophin surge-attenuating factor (GnSAF). METHODS A synthetic single-chain antibody (Tomlinson J) phage display library was bio-panned with partially purified GnSAF produced by cultured human granulosa/luteal...
متن کاملThe recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor.
BACKGROUND Gonadotrophin surge-attenuating factor (GnSAF) is an as yet unidentified ovarian factor that acts on the pituitary to attenuate the pre-ovulatory LH surge. In a previous study, GnSAF bioactivity was proposed to derive, at least in part, from a C-terminal domain (95peptide) of human serum albumin (HSA). METHODS AND RESULTS We employ here the expression-secretion system of Pichia pas...
متن کاملMarja-liisa Hendriks Purification of a Candidate Gonadotropin Surge-inhibiting/attenuating Factor (gnsif/af) Showing Mapk as a Possible Target
Gonadotropin surge-inhibiting/attenuating factor (GnSIF/AF) has been known for over two decades, but its molecular structure has not been completely characterized yet. In the last 20 years, five different putative GnSIF/AF sequences have been published. In this article, we describe a procedure to isolate and characterize GnSIF/AF from bovine follicular fluid. A GnSIF/ AF-derived synthetic pepti...
متن کاملOptimization of The Cell Aggregates Method for Isolation and Purification of Human Granulosa Cells from Follicular Fluid
Objective Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGC) and other contaminating cell types. Several strategies such as the antibody binding methods, the flask method, the cell strainer, and the positive selection of granulosa aggregates after density gradient centrifugation, were tested as LGCs purification methods. Each of these strategies has its own advantages...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Human Reproduction
سال: 1999
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/14.6.1449